Figure 2

uPAR-positive cells in prostate cancer vs. lymph node metastases (200× images). Tumor section images taken from one representative mouse and stained with rabbit anti-uPAR (399R, American Diagnostica Corp.). A. Little or no uPAR staining is apparent in the cancer (Ca) cells within the mouse prostate. Image taken at tumor periphery. B. Strong uPAR immunoreactivity in metastatic cancer cells (Ca) is seen adjacent to lymphatic cells (lym).C. uPAR-positive cells in prostate vs. lymph node (l.n.) metastases in each of 5 mice. Data show the total number of uPAR-positive cells counted in each prostate and l.n. for each mouse. White bars represent primary tumors, black bars represent the l.n. metastases. The data for all 5 mice was collectively analyzed using a paired t-test and the p value was 0.0011.