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Table 3 Sequence analysis of PDGFRA exons 12 and 18 from primary cervical cancer tumors and normal cervix samples.

From: Expression of platelet derived growth factor family members and the potential role of imatinib mesylate for cervical cancer

SAMPLE

EXON 12

EXON 18

 

localization

change

position

amino acid

localization

change

position

amino acid

CU-194

WT

WT

CU-493

WT

Exon

C >T GTC>GTT ht

Codon824

Val > Val

CU-693

WT

WT

CU-794

WT

Exon

C >T GTC>GTT hm

Codon 824

Val > Val

CU-1094

WT

Exon

C >T GTC>GTT ht

Codon 824

Val > Val

CU-1194

WT

Exon

C >T GTC>GTT ht

Codon 824

Val > Val

CU-1494

WT

WT

CU-1593

WT

Exon

C >T GTC>GTT ht

Codon 824

Val > Val

CU-2294

WT

Exon

C >T GTC>GTT ht

Codon 824

Val > Val

CU-3194

WT

Exon

C >T GTC>GTT hm

Codon 824

Val > Val

CU-3293

WT

Exon

C >T GTC>GTT ht

Codon 824

Val > Val

CU-3994

WT

Exon

C >T GTC>GTT hm

Codon 824

Val > Val

CU-4594

WT

WT

CU-4793

WT

WT

CU-4994

WT

WT

CU-5393

WT

WT

CU-5593

WT

Exon

C >T GTC>GTT ht

Codon 824

Val > Val

CN-893

Intron

C > A

IVS12 +22

------

Exon

C >T GTC>GTT ht

Codon 824

Val > Val

CN-993

Intron

C > A

IVS12 +22

-----

Exon

C >T GTC>GTT ht

Codon 824

Val > Val

CN-994

WT

Exon

C >T GTC>GTT ht

Codon 824

Val > Val

HD

WT

WT

  1. WT: wild type; IVS: intronic variation sequence; ht: heterozygous; hm: homozygous. CU = cervical cancer, CN = non-neoplastic cervix, HD = healthy donor lymphocytes. The polymorphisms were presented as ht = heterozygous or hm = homozygous. [RefSeq accession D50017]. All patients were wild type in exon 12, and 10 out of 17 had the silent mutation C >T in codon 824 GTC>GTT, Val>Val [rs10015469]. 2 samples of non-neoplastic cervical tissue presented the intronic variation C>A IVS12+22 [rs2307051] and all 3 presented the silent mutation in codon 824 [rs10015469]. In this table we show the results of only one lymphocytes donor, but all 8 donors were wild type for exon 12, and 3 out of 8 presented the polymorphism at codon 824.