Incubation time | 0 min | 15 min | 30 min | 60 min | 120 min | 180 min | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Experiment no. | II | III | IV | II | III | IV | II | III | IV | II | III | IV | II | III | IV | II | III | IV |
Cytoplasmic fraction (absolute counts, cpm) | 74 883 | 470 559 | 275 481 | 542 304 | 674 216 | 677 588 | 980 421 | 854 765 | 960 000 | 1 060 674 | 518 282 | 863 345 | 368 101 | |||||
% of the amount added to medium | 0.7% | 2.3% | 1.5% | 5.0% | 6.2% | 6.3% | 4.8% | 4.7% | 4.7% | 5.8% | 4.8% | 4.2% | 2.0% | |||||
Amount in μg according to % | 0.0098 | 0.08 | 0.0375 | 0.07 | 0.087 | 0.088 | 0.17 | 0.118 | 0.17 | 0.145 | 0.67 | 0.15 | 0.05 | |||||
Interchromosomal fraction (absolute counts, cpm) | 11 460 | 3060 | 7330 | 83 662 | 95 821 | 97 147 | 159 378 | 322 008 | 299 963 | 445 426 | 177 814 | 282 826 | 203 421 | |||||
% of the amount added to medium | 0.1% | 0.015% | 0.04% | 0.77% | 0.89% | 0.9% | 0.78% | 1.75% | 1.47% | 2.42% | 1.65% | 1.39% | 1.1% | |||||
Amount in μg according to % | 0.0014 | 0.0005 | 0.0025 | 0.01 | 0.013 | 0.0135 | 0.03 | 0.04 | 0.053 | 0.061 | 0.023 | 0.05 | 0.028 | |||||
% of the genome | 0.066% | 0.016% | 0.083% | 0.33% | 0.43% | 0.45% | 1.0% | 1.33% | 1.77% | 2.03% | 0.73% | 1.67% | 0.92% | |||||
Chromatin (absolute counts, cpm) | 8089 | 3691 | 2248 | 45 812 | 47 684 | 49 394 | 296 771 | 123 390 | 242 836 | 157 124 | 37 620 | 244 937 | 118 285 | |||||
% of the amount added to medium | 0.075% | 0.018% | 0.012% | 0.4% | 0.44% | 0.46% | 1.45% | 0.67% | 1.19% | 0.85% | 0.35% | 1.2% | 0.64% | |||||
Amount in μg according to % | 0.0011 | 0.00065 | 0.0003 | 0.0056 | 0.006 | 0.0064 | 0.05 | 0.0168 | 0.0428 | 0.021 | 0.0042 | 0.043 | 0.016 |