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Table 3 Quantitative characteristics of the labeled material content in various cell compartments depending on the time of its incubation with cells

From: Qualitative and quantitative characteristics of the extracellular DNA delivered to the nucleus of a living cell

Incubation time 0 min 15 min 30 min 60 min 120 min 180 min
Experiment no. II III IV II III IV II III IV II III IV II III IV II III IV
Cytoplasmic fraction (absolute counts, cpm) 74 883 470 559 275 481 542 304    674 216    677 588 980 421 854 765   960 000 1 060 674 518 282 863 345 368 101
% of the amount added to medium 0.7% 2.3% 1.5% 5.0%    6.2%    6.3% 4.8% 4.7%   4.7% 5.8% 4.8% 4.2% 2.0%
Amount in μg according to % 0.0098 0.08 0.0375 0.07    0.087    0.088 0.17 0.118   0.17 0.145 0.67 0.15 0.05
Interchromosomal fraction (absolute counts, cpm) 11 460 3060 7330 83 662    95 821    97 147 159 378 322 008   299 963 445 426 177 814 282 826 203 421
% of the amount added to medium 0.1% 0.015% 0.04% 0.77%    0.89%    0.9% 0.78% 1.75%   1.47% 2.42% 1.65% 1.39% 1.1%
Amount in μg according to % 0.0014 0.0005 0.0025 0.01    0.013    0.0135 0.03 0.04   0.053 0.061 0.023 0.05 0.028
% of the genome 0.066% 0.016% 0.083% 0.33%    0.43%    0.45% 1.0% 1.33%   1.77% 2.03% 0.73% 1.67% 0.92%
Chromatin (absolute counts, cpm) 8089 3691 2248 45 812    47 684    49 394 296 771 123 390   242 836 157 124 37 620 244 937 118 285
% of the amount added to medium 0.075% 0.018% 0.012% 0.4%    0.44%    0.46% 1.45% 0.67%   1.19% 0.85% 0.35% 1.2% 0.64%
Amount in μg according to % 0.0011 0.00065 0.0003 0.0056    0.006    0.0064 0.05 0.0168   0.0428 0.021 0.0042 0.043 0.016