Figure 5

Knockdown of HIF-2α has no effect on fibronectin deposition. (A) The amount of fibronectin deposited by 786-O cells stably transfected with VHL (VHLp30 and VHLp19), parental 786-O cells (786-O), and 786-O cells infected with an empty retrovirus (pSuperRetro) or doubly infected with both HIF-2α shRNAs (HIF2α shRNA 1+2) was measured by ELISA at 405 nm. Error bars represent standard deviation. Differences between VHLp30 cells and all other cell lines were statistically significant (P < 0.03). (B) Fibronectin ELISA was performed as in (A) using A498 cells stably transfected with VHL (VHLp19), parental A498 cells (A498), and A498 cells infected with an empty retrovirus (pSuperRetro) or doubly infected with both HIF-2α shRNAs (HIF2α shRNA 1+2). Differences between VHLp19 cells and all other cell lines were statistically significant (P < 0.009).