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Table 1 Genes up-regulated (p < 0.0025 and ≥2 fold-change) after decitabine treatment identified using AffyChip.

From: Modulation by decitabine of gene expression and growth of osteosarcoma U2OS cells in vitro and in xenografts: Identification of apoptotic genes as targets for demethylation

Gene Symbol Fold Change (Affy) Fold Change (Real-Time) CpG known or proposed function
FAM65A 2.76 nd yes hypothetical protein LOC79567
GADD45A 2.58 6 yes Regulation of cyclin dependent protein kinase activity and DNA repair
GAGE4 3.51 nd yes Unknown
HSPA9B 2.32 5.3 yes Implicated in the control of cell proliferation and cellular aging
IGFBP6 2.22 3 yes Insulin-like growth factor binding protein involved in negative regulation of cell proliferation
IMP-3 8.36 nd yes Binds to insulin-like growth factor II leader 3 mRNA and may repress its translation during late development
NFKBIA 4.01 4.2 yes Inhibits NF-k-B and involved in cell adhesion, apoptosis, differentiation and growth
PAWR 3.30 10 yes Pro-apoptotic protein acts by down-regulating the anti-apoptotic protein BCL2 via its interaction with WT1
PDCD5 4.15 8 yes Induction of programmed cell death
PSG5 2.32 nd no Synthesized by syncitiotrophoblast of the placenta and has a role in maintenance of gestation
RAC2 5.09 nd no Involved in the regulation of the NADPH oxidase
TGM2 2.01 nd yes A peptide cross-linking factor
TNFAIP3 3.44 4.5 yes Negative regulation of I-k-B kinase/NF-k-B cascade
  1. Fold change (Affy) is the Mean from a duplicate AffyChip experiments. The fold change using real-time PCR (TaqMan method) is the Mean values from the same RNA from the same yields that were used for the AffyChip experiments and a third replica. The direction of expression was consistent in the Affy experiment and the real-time experiment for the tested genes. 11 out of 13 genes have CpG-island in there 5' region. Genes in bold characters are those identified in the apoptotic pathway as explained in "Methods" and "Results". The genes are listed in alphabetical order.