Inhibition of JNK phosphorylation correlates with treatments that restore Dex-sensitivity to CEM-C1-15 cells. (A) CEM-C1-15 cells were at once treated with vehicle control (C), Dex (D), FSK (F), or the combination of FSK plus Dex (DF) for 24 hours. JNK was evaluated by immunoblot for phosphorylation of Threonine183/Tyrosine185 and total protein, example of n = 4. (B) CEM-C1-15 cells were pretreated with vehicle control (C), U0126 (U) plus SP600125 (SP), rapamycin (R), or combinations of these drugs for 6 hours before adding Dex (D) for an additional 18 hours. JNK was evaluated as in panel A, example of n = 2–4 for various combinations of treatments.