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Table 1 Tunicamycin-induced sensitization of UWOV2 ovarian carcinoma cells to various antineoplastic drugs

From: Combination of tunicamycin with anticancer drugs synergistically enhances their toxicity in multidrug-resistant human ovarian cystadenocarcinoma cells

Drug Alone** Combination with TM** Potency ratio§ (95 % CI; p‡‡) Isobolar analysis of drug combinations
  EC 50 * 95 % CI EC 50 * 95 % CI   Index (I x ) |,∫ Interpretation
DXR 3.95 3.19 to 4.88 0.05 0.03 to 0.06 88 (62 to 126; p < 0.001) 0.15 ± 0.002 (n = 9) Synergism
EXR 2.87 0.76 to 10.80 0.03 0.02 to 0.04 102 (29 to 354; p < 0.001) 0.23 ± 0.002 (n = 7) Synergism
VCR 23.2 9.11 to 59.07 4.60 0.10 to 208 5 (0.14 to 177; p = 0.335) 0.54 ± 0.003 (n = 10) Synergism
CDDP 4.07 3.68 to 4.51 0.24 0.13 to 0.44 17 (10 to 31; p < 0.001) 0.33 ± 0.002 (n = 9) Synergism
  1. *Data (μg/ml) are best-fit values obtained from non-linear regression analysis of the sigmoidal dose-response relation for each drug alone or in combination with a fixed concentration of 5 μg/ml TM. CI, confidence interval; §The potency ratio and associated 95% CI were computed using GraphPad QuickCalcs http://www.graphpad.com according to the method of Fieller [82] by subtracting the log EC50 of drug in combination with TM from the log EC50 of drug alone and back-transformation (antilog) of data; Analysis according to Chou and Talalay [81], using the CombiTool computer programme (version 2.001, http://www.imb-jena.de); Ix, the drug interaction index [73,79,80], |Values are geometric means ± SEM (standard error of the mean) of multiple effect levels for each drug in the dose range 10-4 to 101 μg/ml in the presence of a fixed concentration of 5 μg/ml TM; Ix < 1 synergy; Ix = 1 additivity and Ix > 1 antagonism; ‡‡Obtained from unpaired t-tests for the difference between log EC50 for drug alone and drug in combination with TM; **One-way ANOVA for the differences in log EC50 among treatment groups yielded p < 0.001 in all cases, except for VCR alone vs VCR in combination with TM which showed marginal significance: p = 0.061). Student t-tests to evaluate significant differences in Ix compared to a null hypothesized Ix value of 1, yielded p values < 0.001 in all cases.