Figure 4

Gene expression is impacted by DNMT3b silencing. A. Expression profile obtained after mRNA was isolated from control and DNMT3b silenced PC3 cells, labeled and then hybridized to prostate cancer specific GE-array. B Results of gene expression profile. Only the genes whose expression increased in DNMT3b silenced cells 2-fold, relative to control cells, are listed. C and D. Expression of CDKN3 and cytochrome b5 was analyzed in DNMT3b silenced cells and controls by semi-quantitative RT-PCR.