Figure 4
From: Polyamine depletion induces G1 and S phase arrest in human retinoblastoma Y79 cells
Effects of DFMO on association of p107 and cellular proteins. A: Effects of DFMO on p107/E2F complexes in Y79 cells. Whole cell lysates (100 μg) treated with 5 mM DFMO for 0, 24, 48 h or 5 mM DFMO and 20 μM putrescine for 48 h were immunoprecipitated with anti E2F-4 antibody and immunoblotted with anti p107 anitibody. B: Cell lysates (100 μg) were immunoprecipitated with anti E2F-1, 2, 3, and 5 antibodies and immunoblotted with anti p107 anitibody (upper panels) or with anti E2F-1, 2, 3, and 5 antibodies (lower panels). C: Cell lysates (100 μg) were immunoprecipitated with anti C-Myc antibody and immunoblotted with anti p107 anitibody (left panel). The lysate of Y79 cells treated with DFMO for 24 h were immunoprecipitated with anti C-Myc antibody and immunoblotted with anti C-Myc antibody (right panel). D: Cell lysates (100 μg) were immunoprecipitated with anti p107 antibody and immunoblotted with anti B-Myb anitibody (left panel). The lysate of Y79 cells treated with DFMO for 24 h were immunoprecipitated with anti p107 antibody and immunoblotted with anti p107 antibody (right panel). E: Cell lysates (100 μg) were immunoprecipitated with anti MCM7 antibody and immunoblotted with anti p107 anitibody.