Lack of inhibitory effect on cell proliferation by soluble APP in CM from hAPPs expressing cells. MDA-MB231-L cells, B16F-L cells (500 cells/well), hAPPs and HEK293 cells (1000 cells/well) were seeded in separate 96 well plates. The number of MDA-MB231-L cells and B16F-L cells was measured by BLI assay after incubation of 36 hr-CM from hAPPs (A) or HEK293 cells (B). The media from hAPPs cells showed no significant difference in their ability to inhibit tumor cell proliferation compared to media from control HEK293 cells. All values represent means ± SEM (n = 6).