Immunofluorescence expression of NUCKS and Ki67 in a representative primary culture derived from IDC, grade II. The scarce expression of Ki67 and the ubiquitous expression of NUCKS are demonstrated. (A), cells counterstained with DAPI. (B), NUCKS expression in all of the cell nuclei. (C), only one cell in the optical field expressing Ki67 (arrow). Original magnification ×200.