Quantitative real-time PCR expression profile of miR 17–92 cluster and the host transcript C13orf25 in prostate cancer cell lines. (A) miRNA expression as determined by qRT-PCR. The relative expression of each miRNA is plotted as 2-ΔCt after normalization to sno66 expression. Each bar represents mean ± SEM of at least 3 replicates. (B) qRT-PCR analysis of C13orf25 mRNA expression. Each bar represents C13orf25 mRNA expression normalized to GAPDH mRNA expression. Data are plotted as mean ± SEM of six replicates. (MDA = MDA PCa 2b).