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Table 1 Primers utilized in this investigation

From: Abnormal expression, localization and interaction of canonical transient receptor potential ion channels in human breast cancer cell lines and tissues: a potential target for breast cancer diagnosis and therapy

Primer name

Primer sequence

Expected product size (bp)

TRPC1 F

5'-GTA AGT GGA TTT GCT CTC AT-3'

298 for TRPC1B splice variant

TRPC1 R

5'-TGG TTA ATT TCT TGG ATA AA-3'

 

TRPC3 F

5'-TAC TCA ACA TGC TAA TTG CTA TGA T-3'

383

TRPC3 R

5'-CAC AGT TGC TTG GCT CTT GTC TTC C-3'

 

TRPC4 F

5'-CTC TGG TTG TTC TAC TCA ACA TG-3'

781

TRPC4 R

5'-CCT GTT GAC GAG CAA CTT CTT CT-3'

 

TRPC5 F

5'-GCT CGC AGC CAC CCC AAA GGG AGG A-3'

548

TRPC5 R

5'-CCA ATG TCC CTA CCC TGT TCT CCC AGC TCT C-3'

 

TRPC6 F

5'-GAA CTT AGC AAT GAA CTG GCA GT-3'

625 or 277

TRPC6 R

5'-CAT ATC ATG CCT ATT ACC CAG GA-3'

 

TRPC7 F

5'-GTC CGA ATG CAA GGA AAT CT-3'

477

TRPC7 R

5'-TGG GTT GTA TTT GGC ACC TC-3'

 

TRPM7 F

5'-AAA CCA GTT CTG CCT CCT CCA C-3'

100

TRPM7 R

5'-GTC CAT CGG AAG TCT TAT CTT TCT TTC-3'

 

TRPM8 F

5'-GAT TTT CAC CAA TGA CCG CCG-3'

502

TRPM8 R

5'-CCCCAGCAGCATTGATGTCG-3'

 

TRPV6 F

5'-GAG CAG TCC CTG CTG GAA CT-3'

254

TRPV6 R

5'-GGT ACT TCG AGA CAC TGA GG-3'

 

Cav3.2 F

5'-GAA GGA CAC GCT GCG CGA GT-3'

180

Cav3.2 R

5'-GCA TCC TCC CGT GCC TCC TT-3'

 

β-actin F

5'-ATG GAT GAT GAT ATC GCC GC-3'

350

β-actin R

5'-ATC TTC TCG CGG TTG GCC TT-3'

 

TRPC1 F nested

5'-ATGGCGGCCCTGTACCCGAG-3'

 

TRPC1 R nested

5'-AACACAATGTGCATTCACAG-3'

 

TRPC3 F nested

5'-AAGTGACTTCCGTTGTGCTC-3'

 

TRPC3 R nested

5'-AGGTTGCTGCATCATTCACA-3'

 

TRPC4 F nested

5'-TGTGACCAATGTCAAAGCAC-3'

 

TRPC4 R nested

5'-CTAACACACATTGTTCACTG-3'

 

TRPC5 F nested

5'-CACATCACAACCACGACG-3'

 

TRPC5 R nested

5'-ACTTGCCTGTAACAGATCGG-3'

 

TRPC6 F nested

5'-CAA CCA GAA ACA GAA GCA TG-3'

 

TRPC6 R nested

5'-CTC GCA ATG AAT GAT GCT GC-3'

 

TRPC7 F nested

5'-CACAGTTCTCCTGGACAGAA-3'

 

TRPC7 R nested

5'-TTACTTCAGATAAGCCGAAT-3'

 
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Cancer Cell International

ISSN: 1475-2867

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