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Table 1 Primers utilized in this investigation

From: Abnormal expression, localization and interaction of canonical transient receptor potential ion channels in human breast cancer cell lines and tissues: a potential target for breast cancer diagnosis and therapy

Primer name Primer sequence Expected product size (bp)
TRPC1 F 5'-GTA AGT GGA TTT GCT CTC AT-3' 298 for TRPC1B splice variant
TRPC1 R 5'-TGG TTA ATT TCT TGG ATA AA-3'  
TRPC3 F 5'-TAC TCA ACA TGC TAA TTG CTA TGA T-3' 383
TRPC3 R 5'-CAC AGT TGC TTG GCT CTT GTC TTC C-3'  
TRPC4 F 5'-CTC TGG TTG TTC TAC TCA ACA TG-3' 781
TRPC4 R 5'-CCT GTT GAC GAG CAA CTT CTT CT-3'  
TRPC5 F 5'-GCT CGC AGC CAC CCC AAA GGG AGG A-3' 548
TRPC5 R 5'-CCA ATG TCC CTA CCC TGT TCT CCC AGC TCT C-3'  
TRPC6 F 5'-GAA CTT AGC AAT GAA CTG GCA GT-3' 625 or 277
TRPC6 R 5'-CAT ATC ATG CCT ATT ACC CAG GA-3'  
TRPC7 F 5'-GTC CGA ATG CAA GGA AAT CT-3' 477
TRPC7 R 5'-TGG GTT GTA TTT GGC ACC TC-3'  
TRPM7 F 5'-AAA CCA GTT CTG CCT CCT CCA C-3' 100
TRPM7 R 5'-GTC CAT CGG AAG TCT TAT CTT TCT TTC-3'  
TRPM8 F 5'-GAT TTT CAC CAA TGA CCG CCG-3' 502
TRPM8 R 5'-CCCCAGCAGCATTGATGTCG-3'  
TRPV6 F 5'-GAG CAG TCC CTG CTG GAA CT-3' 254
TRPV6 R 5'-GGT ACT TCG AGA CAC TGA GG-3'  
Cav3.2 F 5'-GAA GGA CAC GCT GCG CGA GT-3' 180
Cav3.2 R 5'-GCA TCC TCC CGT GCC TCC TT-3'  
β-actin F 5'-ATG GAT GAT GAT ATC GCC GC-3' 350
β-actin R 5'-ATC TTC TCG CGG TTG GCC TT-3'  
TRPC1 F nested 5'-ATGGCGGCCCTGTACCCGAG-3'  
TRPC1 R nested 5'-AACACAATGTGCATTCACAG-3'  
TRPC3 F nested 5'-AAGTGACTTCCGTTGTGCTC-3'  
TRPC3 R nested 5'-AGGTTGCTGCATCATTCACA-3'  
TRPC4 F nested 5'-TGTGACCAATGTCAAAGCAC-3'  
TRPC4 R nested 5'-CTAACACACATTGTTCACTG-3'  
TRPC5 F nested 5'-CACATCACAACCACGACG-3'  
TRPC5 R nested 5'-ACTTGCCTGTAACAGATCGG-3'  
TRPC6 F nested 5'-CAA CCA GAA ACA GAA GCA TG-3'  
TRPC6 R nested 5'-CTC GCA ATG AAT GAT GCT GC-3'  
TRPC7 F nested 5'-CACAGTTCTCCTGGACAGAA-3'  
TRPC7 R nested 5'-TTACTTCAGATAAGCCGAAT-3'