Figure 3
Morphological studies by inverted microscope at actual magnification 100×. HepG2 and non-malignant Chang's liver cell line were treated without (A, C) and with 12.5 μg mL-1 of P. sarmentosum ethanolic extract (B, D) for 72 hours. Both types of treatment (C and D) produced similar cellular morphology and antiproliferative effect. However, in HepG2 cells, the confluency appeared to be reduced from 90% in untreated cells to 10% in treated cells. Similar cellular morphology was observed in three independent experiments (n = 3).