Figure 6

Morphological observation with acridine orange and ethidium bromide (AO/EB) staining at actual magnification 400×. HepG2 cells were treated without (A) and with P. sarmentosum ethanolic extract, 10 μg mL^-1 (B), 12 μg mL^-1 (C) and 14 μg mL^-1 (D) for 72 hours. Dashed arrow indicated cells with chromatin condensation; rounded dotted arrow indicated cells with fragmented nuclei; dashed dotted arrow indicated cells with membrane blebbing and full white arrow indicated the presence of apoptotic bodies. Each experiment was performed in triplicate (n = 3) and generated similar morphological features.