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Figure 8 | Cancer Cell International

Figure 8

From: Increasingly transformed MCF-10A cells have a progressively tumor-like phenotype in three-dimensional basement membrane culture

Figure 8

Morphology observed for MCF-10A, MCF-10AT, and MCF-10CA1a cells grown in three-dimensional culture under varying conditions. Phase contrast images of MCF-10A (A, B), MCF-10AT (C, D) and MCF-10CA1a (E, F) grown in overlay conditions at a low (0.5%) concentration of Matrigel. MCF-10A cells form spherical acini; MCF-10AT transformed cells form long, branched structures that thicken between days 6 and 20. MCF-10CA1a cells form interconnected masses of cells. Phase contrast images of MCF-10CA1a cells grown in overlay rBM in complete (G) and minimal (H) media. MCF-10CA1a cells at day 18 in 3D culture appear less dense and more spread under the minimal media conditions. Scale bars, 100 μm.

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Cancer Cell International

ISSN: 1475-2867

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