Figure 4
From: In vitro assessment of antitumor activities of the PI3K/mTOR inhibitor GSK2126458
Invasive ductal carcinoma. (a-f) Histology and expression of caspase-3 by immunoperoxidase. (a-b) Untreated tumor cords and nests of neoplastic cells in a background of desmoplastic reaction, H&E at 10× and 40×, respectively. (d-e) Treated tumor demonstrating decreased cellularity, cellular dyscohesion and numerous apoptotic bodies (black arrow), H&E at 10× and 40×, respectively. (c and f) demonstrating rare expression by caspase-3 in untreated tumor ( 1%) compared to up to 2% in treated tumor, immunoperoxidase (IP), 40×. (g) HPLC runs of intracellular caspase-3 activity in treated and untreated tumor (AMC peak retention time, ~4.8 min). (h) Cellular respiration, measured immediately on tumor arrival to the laboratory to affirm viability.