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Figure 1 | Cancer Cell International

Figure 1

From: Increase of IFN-γ and TNF-γ production in CD107a + NK-92 cells co-cultured with cervical cancer cell lines pre-treated with the HO-1 inhibitor

Figure 1

Expression of Heme oxygenase 1 (HO-1) in different Cervical cancer cell (CCC) lines. Expression of HO-1 in HeLa, SiHa, and C-33A cells was detected by indirect staining protocol using a PE-conjugated anti-mouse secondary antibody after incubation with mouse anti-HO-1 primary antibody. Results represent the mean ± Standard deviation (SD) of three independent experiments carried out in triplicate. A representative experiment of HO-1 expression in HeLa, SiHa, and C-33A cells is shown (a). Percentage of HO-1 expression in HeLa, SiHa, and C-33A cells (b). After treatment with HO-1 inhibitors, viability in HeLa, SiHa, and C-33A cells was evaluated with Sytox by Flow cytometry (FC) (c). Expression of MICA and MICB in HeLa, SiHa, and C-33A cells treated or not with SnPP (25 µM) or ZnPP (1 µM), (HO-1 inhibitors) (d). *P <0.05 HeLa, SiHa vs. C-33A cells.

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