Figure 5

Autophagy and cell-cycle regulation of OC3/P cells treated with SAHA or/and PTX. A: Cells were pretreated with 16 μM SAHA for 24 h and then cultured with 2 μM PTX for a further 24 h, or were treated with serum-free 1640 for 24 h followed by PTX 1640 dilution for 24 h. Cell morphology and structure were viewed by transmission electron microscopy. B: Cells were treated with 4, 16 and 64 μM SAHA for 24 h followed by serum-free 1640 for 24 h. Cell morphology and structure were viewed by transmission electron microscopy. The arrows indicate autophagosomes. N, nucleus; M, mitochondria. C: OC3/P cells were treated with SAHA, or PTX, or a combination of both as described previously (methods as above). The fractions of cells in G1-phase, S-phase and G2/M phase were determined by flow cytometry.