Figure 3
From: The immunoglobulin heavy chain VH6-1 promoter regulates Ig transcription in non-B cells
The sequence 1200 bp to 300 bp upstream of VH6-1 contains no regulatory elements. (A) Schematic diagram of 5′-flanking 300-bp pGL3 construct. (B) The 300-bp deletion fragment amplified from the 1.2-kb fragment containing the VH6-1 promoter by PCR. (C) The 300-bp deletion truncations of the 1.2-kb pGL3 construct exhibited similar promoter activity to the 1.2-kb pGL3 construct in HT-29 and Raji cells.