PI3K-AKT signaling is required for M. hyorhinis infection and induced cell migration in MGC803 cells. (A) AG1478 or wortmannin pretreatment inhibits M. hyorhinis infection. AG1478 or wortmannin was added in cell medium 1 hour prior to M. hyorhinis exposure. Then the cells were subjected to PCR amplification of p37. (B) and (C) AG1478 or wortmannin abolishes M. hyorhinis (B) and GST-p37 (C) induced cell migration. Transwell cell migration assay was performed according to manufacturer’s protocol. Values represented the mean ± SD from three to four independent experiments with triplicate samples. *, P <0.05.