Figure 5

BIX-01294 triggers ER stress response in human bladder cancer cells. (A) T24 and 5637 cells were treated with indicated concentrations of BIX-01294 for 24 hours and harvested for Western blot analysis. Levels of protein expression were analyzed by using antibodies against HSPA5, ERN1, ATF4 and ACTB. (B) T24 and 5637 cells were treated with 10 μmol/l of BIX-01294 for various lengths of time. Both attached and suspended cells were harvested for Western blot analysis by using antibodies against HSPA5, ERN1, ATF4 and ACTB.