Figure 3

miR-191 was functionally involved in the radiation resistance to A549 and H1299 cells. (A) Micro-RNA profiles of radiation resistant and non radiation resistant A549 and H1299 cells were shown. Supervised hierarchical clustering of cell lines based on their differential miRNAs expression with ΔLMR > 2 between the two groups were exhibited. Each column represents a cell line and each row a probe set. (B-C) Relative miRNAs expression in A549 and H1299 cells in (A). (D) Downregulation of miR-191 expression in radiation resistant A549 compared to non-radiation resistant A549 cells. Results were normalized to snU6 expression level and represented as mean ± S.E. from three independent replicates. (*p <0.05 vs control, n = 3). (E) Downregulation of miR-191 expression in radiation resistant H1299 compared to non-radiation resistant H1299 cells. Results were normalized to snU6 expression level and represented as mean ± S.E. from three independent replicates. (*p <0.05 vs control, n = 3). (F) Cell viability after infected with Lenti-virus-miR-191 for 48 h.