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Table 3 Purification scheme for LDH in normal breast samples

From: Kinetic characterization of lactate dehydrogenase in normal and malignant human breast tissues

Purification step Total protein (mg) Total activity (U) Specific activity (U/mg) Fold purification % yield
Supernatant 8.5 2.4 0.28 - -
DEAE-Sephadex 1.9 1.35 0.71 2.5 56
Blue Sepharose CL-6B 0.18 0.42 2.3 8.2 17