Table 3 Purification scheme for LDH in normal breast samples
From: Kinetic characterization of lactate dehydrogenase in normal and malignant human breast tissues
Purification step | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Fold purification | % yield |
|---|---|---|---|---|---|
Supernatant | 8.5 | 2.4 | 0.28 | - | - |
DEAE-Sephadex | 1.9 | 1.35 | 0.71 | 2.5 | 56 |
Blue Sepharose CL-6B | 0.18 | 0.42 | 2.3 | 8.2 | 17 |