Figure 2
From: Human urothelial carcinoma cell response to Sunitinib malate therapy in vitro
Apoptosis analysis by flow cytometry. A, flow cytometric analysis of propidium iodide stained DNA of three bladder cancer cell lines following Sunitinib treatment. Cells were treated with Sunitinib for 48 h for indicated concentration. Increased sub-G1 fraction the Sunitinib -treated cells provided an estimate of apoptotic cells. B, Apoptosis detected by Annexin V-FITC/PI binding assay. Cells treated with Sunitinib for 48 h were stained with Annexin V and PI before subjected to FACS for analysis.