Figure 2

Protein c-MYC binds to the promoter of BCYRN1. A/B. Protein (A) and mRNA (B) levels of BCYRN1 and c-MYC in the NSCLC cells infected with pWPXL. 10058-F4: a small-molecule c-MYC inhibitor; groups shRNA-c-MYC, shRNA-NC (NC: negative control), pWPXL-c-MYC and pWPXL-NC respectively represented the groups where NSCLC cells were transfected with specific shRNA against c-MYC, non-specific shRNA, and infected with pWPXL-c-MYC vector or the pWPXL vector containing non-specific sequence. (C) ChIP assay showed binding of c-Myc to the BCYRN1 promoter in NSCLC tissues. (D) ChIP assay showed binding of c-Myc to the BCYRN1 promoter in A549 cells. Soluble chromatin of A549 cells and NSCLC tissue were immunopurified using anti-c-MYC antibody and quantitative real-time PCR was used to measure the level of enrichment on BCYRN1. ** p < 0.01, indicated a very significant difference, compared with negative control.