Figure 4
Expression of NKκB, pIκB, PI3K, Akt and pAkt in human colorectal and breast cancer cells treated with Nar. Colorectal (SW1116) and breast (HTB26) cancer cells (2.5 × 105 cells/well) were plated into 24-well plates and incubated at 37°C for 18 h. The cells were then treated with Nar (3 mM) for 24 h. Expression levels of NFκB/pIκB, in the nuclear extracts, (Aa, b and Ba, b); PI3K/pAkt/Akt (Ac-e and Bc- e) and β-actin (Af, Bf), in the whole cell extracts, were detected by western blot analyses. The relative protein expression of NFκB/pIκB (C a, b) and PI3K, pAkt, Akt, pAkt/Akt (Cc- f) was normalized to β-actin. *P ≤ 0.05 vs. control (untreated). The data were obtained from at least duplicate experiments.