Figure 1

Real-time PCR array design. (A) Amplification of target genes and glyceraldehyde 3-phosphate dehydrogenase in real-time PCR arrays. Reactions were run on a Lightcycler 480 (Roche) using = universal thermal cycling parameters: 95°C 5 minutes, 45 cycles of 10 seconds at 95°C, 20 seconds at 60°C, and 15 seconds at 72°C. (B) Melting curve analysis of the PCR product of a target gene. Melting curves were obtained using the cycling parameters: 10 seconds at 95°C, 60 seconds at 60°C, and continuous melting. (C) Amplification of all genes in the PCR array.