Fig. 3

Targeting site of miR-545 in the Ku70 3′UTR. a Ku70 3′UTR was predicted a binding site for hsa-miR-545 and mRNA sequence is shown with potential binding sites indicated in white. The highly conserved mature miR-545 sequence in mammals and potential binding between the miR-545 seed region to the mouse Ku70 3′UTR sequence are shown. 24 h after transfected with miR-454 mimic, Lewis lung carcinoma cells were lyzed to examine (b) miR-454 expression by using quantity RT-PCR, (c) Ku70 3′UTR activity using luciferase report, (d) Ku 70 mRNA level by using quantity RT-PCR and (e) Ku 70 protein expression by using western blot assay. 24 h after transfected with miR-454 inhibitor, Lewis lung cancer cells were lyzed to examine (f) miR-454 level, (g) Ku70 3′UTR activity, (h) Ku 70 mRNA level and (i) Ku 70 protein expression by using the same detection as before. The results were represented as mean ± SD. ^* P < 0.05 compared with negative control