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Fig. 1 | Cancer Cell International

Fig. 1

From: RB mutation and RAS overexpression induce resistance to NK cell-mediated cytotoxicity in glioma cells

Fig. 1

Characterization of in vitro transformed astrocytes. (a) Morphological changes of astrocytes stained with violet crystal, (b) expression of GFAP and GFP in transformed astrocytes, by immunofluorescence, (c) expression of pRb, p53, p-p53, RasV12 and p-H2AX, by Western blot with specific antibodies, (d) cell senescence, as assessed by the percentage of SA-β-galactosidase positive cells, (e) cell proliferation rate, as assessed by violet crystal violet uptake. All images are representative of at least three independent experiments

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Cancer Cell International

ISSN: 1475-2867

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