Fig. 2
From: GSK3β mediates pancreatic cancer cell invasion in vitro via the CXCR4/MMP-2 Pathway
Inhibition of GSK3β downregulates CXCR4 and MMP2 expression. PANC1 and SW-1990 human pancreatic cancer cells were transfected with the GSK3β silencing plasmid pcDNA6.2-GW/EmGFP-miR-GSK3β expression vector, or scrambled vector as a control, and stable cell clones were selected. Total cell lysates were isolated and subjected to Western blotting using anti-human GSK3β, CXCR4 and MMP-2 antibodies; α-tubulin was used as a loading control. a GSK3β, CXCR4 and MMP-2 protein expression in PANC1 cells. (C) GSK3β, CXCR4 and MMP-2 protein expression in SW-1990 cells. b and d Quantification of GSK3β, CXCR4 and MMP-2 expression in a and c based on gray-scale analysis. Results are expressed as the mean ± SD; *p < 0.05 compared to control cells