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Fig. 5 | Cancer Cell International

Fig. 5

From: MicroRNA-24 inhibits growth, induces apoptosis, and reverses radioresistance in laryngeal squamous cell carcinoma by targeting X-linked inhibitor of apoptosis protein

Fig. 5

Effects of XIAP knockdown on growth, colony formation, and apoptosis in LSCC cells. a Western blot of XIAP protein expression in Hep-2 and AMC-HN-8 cells stably transfected with pSil/shXIAP or pSil/shcontrol, respectively. GAPDH was used as an internal control. b MTT analysis of growth in Hep-2 and AMC-HN-8 cells stably transfected with pSil/shXIAP or pSil/shcontrol, respectively. c Colony formation assay was performed as described in Methods. d Flow cytometric detection of apoptosis in Hep-2 and AMC-HN-8 cells stably transfected with pSil/shcontrol or pSil/shXIAP, respectively. e Western blot detection of c-caspase-3, total caspase-3, c-PARP, and total PARP proteins in the stably transfected Hep-2 and AMC-HN-8. GAPDH was used as an internal control. Each experiment was performed at least in triplicate. *P < 0.05, **P < 0.01 vs control

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Cancer Cell International

ISSN: 1475-2867

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