Up-regulation of S100A7 protein and mRNA during detachment of anoikis resistant cell lines and in orthotopic mice model. a Both the attached and detached at different time points of cell lysates of TU167 and JMAR were analyzed by western blot for S100A7. β-actin was used as a loading control. b Detection of S100A7 in JMAR detached cells by immunofluorescence after staining with anti-S100A7. c TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labelling) assays were performed to study increasing anoikis resistance cells. d Northern blot showing expression of S100A7 mRNA in TU167 and various clones of JMAR in both attached and detached condition for indicated time intervals. e IHC of S100A7 expression for FFPE tumor sections of mice generated from orthotopic injection of TU167 and JMAR cell lines.