Fig. 5

MiRNA-21 ASO altered the transduction of AKT and ERK pathways in human colon carcinoma cells. Human colon carcinoma cell line HCT116 cells were transiently transfected with p-miR-21-ASO or p-Cont (5 μg). 48 h later, the level of total and phosphor-AKT and phosphor-ERK1/2 were analyzed by Western blotting (a) and calculated (b). One representative of three experiments was shown.*p < 0.05.