Fig. 3

MiR-30b inhibits NSCLC cells migration and invasion. a After transfection of miR-30b mimics or Scramble, qRT-PCR results showed miR-30b expression was significantly increased in miR-30b group compared with NHBE group, Scramble and Blank groups (P < 0.05). b Wound healing assays were used to assess the migration ability of A549 and Calu-3 cells after transfection. miR-30b group showed a lower cell density at 24 h post-wounding compared with both Scramble and Blank groups (P < 0.05). c Transwell migration assays were used to assess the migration ability of A549 and Calu-3 cells after transfection. The numbers of A549 and Calu-3 cells in the miR-30b group passing through membrane were significantly lower compared with those from the Scramble and Blank groups (P < 0.05). d Transwell invasion assays were used to assess the invasion n ability of A549 and Calu-3 cells after transfection. The numbers of A549 and Calu-3 cells in the miR-30b group passing through the membrane and matrigel were significantly lower compared with those from the Scramble and Blank groups (P < 0.05).e, f Western blotting was used to analyze the expression of Cthrc1 and some other invasion-related proteins such as Cthrc1, TIMP-1, MMP-2 and MMP-9 in A549 and Calu-3 cells. The results showed that Cthrc1, MMP-2 and MMP-9 were declined but TIMP-1 was increased following transfection with miR-30b in A549 and Calu-3 cells (P < 0.05). miR-30b cells transfected with miR-30b mimics; Scramble cells transfected with scrambled miR-30b negative control; Blank cells with non-transfected