Fig. 5

Expression of Cthrc1 restores miR-30b anti-invasion function. a, b Western blotting arrays results the Cthrc1 expression of A549 and Calu-3 cells was lower after transfection with miR-30b mimics, and was higher after transfection with miR-30b inhibitor. However, Co-transfection of miR-30b mimics and pcDNA3.1-Cthrc1 led to increased expression of Cthrc1, and abrogated the Cthrc1 expression-reducing effect of miR-30b mimics. c, d Trans-well arrays results the mean number of cells penetrating the trans-well membrane and matrigel decreased increased after transfection with miR-30b mimics, but increased after transfection with pcDNA3.1-Cthrc1 and co-transfection with pcDNA3.1-Cthrc1 and miR-30b mimics, abrogating the effect of miR-30b mimics