Fig. 3
From: Cyclin D3 predicts disease-free survival in breast cancer
Cyclin D3 was involved in the metastasis of breast cancer. MDA-MB231 cells were transfected with siRNA targeting Cyclin D3 or control vectors. After 6 h, transwell assays were performed as described. Crystal violet staining of migrating and invading cells is shown. Data are expressed as the mean ± SEM of the number of invading cells in more than five separate areas. *p < 0.05 versus vector controls (n = 3 experiments). The efficiency of knock down was detected by western blot. GAPDH was used as a loading control