Fig. 2

Anti-proliferative effect of CLN isomers on murine macrophage-like leukemia cells. a, b The murine macrophage-like PU5-1.8 cells were incubated with the solvent control (0.01 % ethanol) or different concentrations of CLN isomers at 37 °C for 48 h. Cell growth was determined by the MTT reduction assay and the results were expressed as mean % inhibition of cell proliferation ± SE. c, d PU5-1.8 cells were incubated with the solvent control (0.01 % ethanol) or different concentrations of jacaric acid at 37 °C for various periods of time. Cell proliferation was determined by the MTT reduction assay (c) or CyQUANT^® NF Cell Proliferation Assay Kit (d) and the results were expressed as mean % inhibition of cell proliferation ± SE. e The murine macrophage-like J774 A.1 and P388D1 cells were incubated with the solvent control (0.01 % ethanol) or different concentrations of jacaric acid at 37 °C for 48 h. Cell growth was determined by the MTT reduction assay, and the results were expressed as mean % inhibition of cell proliferation ± SE