Fig. 6
Induction of DNA fragmentation and modulation of apoptosis-regulatory proteins in jacaric acid-treated PU5-1.8 cells. a PU5-1.8 cells were incubated with different concentrations of jacaric acid at 37 °C for 24 or 48 h. Cells treated with ethanol acted as the control. DNA fragments were detected by using the Cell Death Detection ELISAPLUS Kit. The extent of apoptosis in the samples was expressed as the enrichment factor. b PU5-1.8 cells were incubated with 4 μM jacaric acid (Lane 2) and 8 μM jacaric acid (Lane 3) at 37 °C for 72 h. Cells treated with ethanol (Lane 1) acted as the control. Protein expression levels of Bax, Bcl-2 and Bcl-xL were assayed by Western blotting with β-actin protein as an internal control. c–e The relative protein expression levels of Bax, Bcl-2 and Bcl-xL compared to β-actin were quantified. Results represent mean ± SE. **p < 0.01; ***p < 0.001