Fig. 3
From: STAT1 inhibits human hepatocellular carcinoma cell growth through induction of p53 and Fbxw7
Effect of STAT1 on p53, Fbxw7, Hes-1 and NF-κB p65. a, b, c, d, e Western blot was used to analyze p53, Fbxw7, Hes-1 and NF-κB p65 protein. Actin served as internal control. p53 and Fbxw7 were significantly increased, Hes-1 and NF-κB p65 were significantly decreased in STAT1-transfected SMMC7721 and HepG2 cells compared to SMMC7721, HepG2 and EV cells (P < 0.05); f, g, h, i, j showed p53, Fbxw7, Hes-1 and NF-κB p65 protein expression in STAT1 siRNA2, control siRNA, SMMC7721 and HepG2 cells. The protein of p53 and Fbxw7 were significantly decreased, Hes-1and NF-κB p65 were significantly increased in STAT1 siRNA2 cells compared to control siRNA, SMMC7721 and HepG2 cells (P < 0.05)