Fig. 1
The blood-cerebrospinal fluid barrier (BCSFB) in vitro model (a) used for transmigration experiments (b) and effect of the transmigration process on the barrier function (c, d). a The BCSFB model is based on the culture of human choroid plexus papilloma cells (HIBCPP cells) on the lower surface of the inserts, so that the upper side mimics the blood compartment and the lower one the CSF compartment. For the transmigration experiment, neuroblastoma cells were dissociated and intracellularly stained using BCECF-AM (1), before being seeded in the upper compartment (2). At the end of the experiment (3), the number of transmigrated cells was assessed by fluorescence measurements, and the barrier integrity was evaluated by measuring the trans-epithelial electrical resistance (TEER) and the permeability of the barrier to Lucifer Yellow (LY, integrity marker). b Percentage of transmigrated neuroblastoma cells (having reached the lower compartments after crossing filters alone, or filters + HIBCPP layer), as assessed by fluorescence measurements. c TEER values (in Ω cm2) before and after the transmigration of IMR32 and SH-SY5Y cells. d Permeability coefficient (Pe, in cm/min) for LY measured on control filters and after the transmigration of IMR32 and SH-SY5Y cells. N.S. non significant