Fig. 5

Peel polyphenolic extract inhibits VEGF-A expression in human colon cancer LS174 cells. LS174 cells were treated with vehicle or Peph (5 µg/ml) for 72 h. a The amount of VEGF-A mRNA transcript was quantified by real time PCR. The values are normalized to 36B4 and the control value was taken as 100 %. b Supernatants from LS174 cultured in the absence (vehicle) or presence of Peph (5 µg/ml) were collected and analyzed by Human VEGF specific ELISA. Results are reported as the mean ± SE of three independent experiments each run in triplicate (*p < 0.05). The data were corrected to the cell number