Fig. 3

Different expression of mTOR activity related proteins and Akt in HT-1080 and ZR-75.1 cells. phospho-mTOR (p-mTOR—the active mTOR kinase), phospho-S6 (p-S6—mTORC1 activity related phospho-protein), Rictor (mTORC2 specific protein), Akt and anti-phospho-(Ser473)-Akt expression in HT-1080 and ZR-75.1 cells detected by Western blot. The higher amount of p-S6 with low Rictor expression in HT-1080 suggest that mTORC1 activity is high in HT-1080 and lower in ZR-75.1 cells. mTOR activity could be released mainly in mTORC2 complex in ZR-75.1 cells (low level of p-S6 and high level of Rictor and phospho-(Ser473)-Akt)