Skip to main content

Table 3 Primers used for pyrosequencing

From: ELF5 and DOK7 regulation in anti-estrogen treated cells and tumors

Target Product size (BP) Pyrosequencing primer sequences (5′–3′)
ELF5 230 F: 5′-GTT TGT AGG GTA GGG GTG AGT T-3′
R: 5′-BIOTIN-ACA AAC CCT CCC AAC ACC A-3′
SEQ: 5′-TAA GGA GTAGTG TTA TAT TG-3′
DOK7 265 F: 5′- GGG AAG TAG GAT TGT TTG AAG ATT -3′
R: 5′- BIOTIN-CCA ACC AAA CTC CTT CTC CTA-3′
SEQ: 5′-GTT TTT GTT TTT TGA AAA AG-3′