Table 3 Primers used for pyrosequencing
From: ELF5 and DOK7 regulation in anti-estrogen treated cells and tumors
Target | Product size (BP) | Pyrosequencing primer sequences (5′–3′) |
|---|---|---|
ELF5 | 230 | F: 5′-GTT TGT AGG GTA GGG GTG AGT T-3′ R: 5′-BIOTIN-ACA AAC CCT CCC AAC ACC A-3′ SEQ: 5′-TAA GGA GTAGTG TTA TAT TG-3′ |
DOK7 | 265 | F: 5′- GGG AAG TAG GAT TGT TTG AAG ATT -3′ R: 5′- BIOTIN-CCA ACC AAA CTC CTT CTC CTA-3′ SEQ: 5′-GTT TTT GTT TTT TGA AAA AG-3′ |