Fig. 4

The assessment of the nuclear morphology using DAPI staining and fluorescence microscopy. The A549 cells were incubated with 10 µM fisetin (FIS), 0.1 µM paclitaxel (PTX) either as single agents or in the combination for 6, 10 or 30 h. Micrographs are representative of three independent experiments. Arrowheads indicate: (I) the shrunken or fragmented nuclei with chromatin condensation; the enlarged multinucleated (II) or mononucleated (III) cells. Bar 50 µm