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Fig. 9 | Cancer Cell International

Fig. 9

From: Paclitaxel and the dietary flavonoid fisetin: a synergistic combination that induces mitotic catastrophe and autophagic cell death in A549 non-small cell lung cancer cells

Fig. 9

The detection of autophagy by LC3 accumulation. ao Fluorescent microscope was used to visualize the LC3-II punctate staining pattern (green); the cell nuclei were counterstained with DAPI (blue). al The A549 cells were treated with 10 µM fisetin (FIS) and/or 0.1 µM paclitaxel (PTX) for 24 h or left untreated (control, CTRL). mo As negative control, bafilomycin A1 (Baf A1; 100 nM) was added to the cells for a period of 4 h, followed by washing with PBS and subsequent incubation with FIS and PTX for 24 h. Note the increase in LC3-II puncta following FIS and PTX treatment in the absence or presence of Baf A1 (j, m). Bar 50 µm. p Real-time PCR measurement of LC3-II mRNA expression in A549 cells. The expression was normalized to glucose 6-phosphate dehydrogenase (G6PD) and presented as a fold difference relative to a calibrator sample (untreated A549 cells; designated as 1). Error bars represent standard deviation from duplicate qRT-PCR assays

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