Fig. 4From: Non-DHFR-mediated effects of methotrexate in osteosarcoma cell lines: epigenetic alterations and enhanced cell differentiationEffect of MTX on histone H3 acetylation in OSA-06 cells. Cells were incubated with 1 or 40 μM MTX, VAL or BUT. At day 3, nuclear protein extracts were harvested and immunoblotted by anti-acetyl-histone H3 antibody. PCNA served as a loading controlBack to article page