Fig. 6

Changes in matrix mineralization in osteosarcoma cell lines after treatment with MTX. Saos-2 (a), OSA-02 (b), OSA-03 (c), OSA-05 (d), OSA-06 (e) and OSA-08 (f) cell lines treated with MTX and/or ATRA were measured using staining with Alizarin Red S at day 21 of incubation. The extent of mineralization is presented as a percentage change compared to the levels found in untreated control cells. Untreated controls were set as 100 %. The data represent the mean ± SD. Experiments were repeated three times. *P < 0.05, **P < 0.01, indicate significant differences from the respective control groups