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Table 2 Primers used for detection of mRNA Expression by RT-PCR

From: Knockdown of PSCA induces EMT and decreases metastatic potentials of the human prostate cancer DU145 cells

Gene

Sequences

Forward

Reverse

Human experiments

 PSCA

GCTGCTTGCCCTGTTGATGG

ACATGGTCAGACTTGCGTTAGGA

 GAPDH

CCATCACTGCCACCCAGAAGAC

GTGTCGCTGTTGAAGTCAGAGGAGA

 E-cadherin

TCCTCCCAATACATCTCCCTTCA

TCTCCGCCTCCTTCTTCATCATA

 Vimentin

TTCGCCAACTACATCGACAAGG

TTCAAGGTCAAGACGTGCCAG

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Cancer Cell International

ISSN: 1475-2867

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