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Fig. 3 | Cancer Cell International

Fig. 3

From: Combined EGFR- and notch inhibition display additive inhibitory effect on glioblastoma cell viability and glioblastoma-induced endothelial cell sprouting in vitro

Fig. 3

EC cell sprouting stimulation by GBM-conditioned cell culture media. CPH036 and CPH047 cells were grown for 14 days and the conditioned media was collected and concentrated. EC were grown in presence of 20 ng/ml VEGF or conditioned media from CPH036 or CPH047 cells. a Relative VEGF expression analyzed by Q-RT-PCR, and VEGF secretion (ng/ml) analyzed by ELISA of CPH036 and CPH047 cells (mean ± SEM, n = 6 for Q-RT-PCR and n = 3 for ELISA). b Representative pictures showing sprouting of EC upon stimulation with VEGF or conditioned media from either CPH036 or CPH047 cells. c, d EC sprouting quantified as number of sprouts and total length of sprouts per sphere, respectively, and displayed relative to NB unconditioned media (mean ± SEM, n = 3 measuring an average of 10 spheres per condition in each experiment). Scale bar shows 100 μm. ***p < 0.001 vs. NB medium

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